Cat. №: Р053
2 277,00 ₽
0,00 ₽
+
Ready to use density gradient medium for isolating of PBMC out of fresh sample material.  It was desinged to isolate cells with a density of less than 1,077 g/cm³ from human whole...
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Ficoll solution

Ready to use density gradient medium for isolating of PBMC (peripheral blood mononuclear cells - containing lymphocytes and monocytes) out of fresh sample material. It was desinged to isolate cells with a density of less than 1,077 g/cm³ from human whole blood. Antibiotics-free.

This product is intended for research purposes. The product has a Russian Registration Certificate as a medical product.

  • Appearance: clear solution, without opalescence and sediment. 
  • pH: 7.0 to 7.5
  • Osmolarity: 280±20 mOsm/kg
  • Buffer capacity: at least 1.0 ml
  • Endotoxin content: ≤0.125 EU/ml

PREPARATION INSTRUCTIONS: The medium is ready to use. Manipulate the product in aseptic conditions.

METHOD: 

  1. For lymphocytes isolation whole blood is diluted in a ratio of 1:1 with buffer solution of PBS Ca²⁺, Md²⁺ free (or solution of DPBS Ca²⁺, Md²⁺ free).
  2. The diluted blood is added to Ficoll solution (at the rate of 1.5 volumes of blood per 1 volume of Ficoll solution)/ Avoid a mixing of the two phases.
  3. Centrifugate for 30 minutes at 400 g at room temperature (18-20 °C).
  4. Carefully remove the PBMCs from the density gradient medium: whitish meniscus between a plasma and medium.
  5. The cells are collected and washed in nutrient media for further cultivation and subsequent use for laboratory purposes.

HOW SUPPLIED: in sterile plastic bottle of 100 or 500 ml. Euroflasks have a wide neck, convenient for work.

SHELF LIFE: 24 months. Shelf life after opening the primary packaging: 30 days.

STORAGE: +4 to +30 °C in a place protected from light. After opening the primary packaging, store at temperature of +2 to +8 °C in a place protected from light.

RECOMMENDED USE:

  • Do not use the product after its expiry date.
  • Use gloves, mask and clothes adapted to the manipulation of the product to avoid contamination.
  • Manipulate the product in aseptic conditions. 
  • Supplements such as antibiotics or L-Glutamine should be added as sterile supplements to the ready medium.
  • Medium should be clear and free of opalescence and sediment. 
 
  • Sterility +
  • Solution: Ficoll
  • Documents: Registration Certificate
  • Manufacturer: PanEco
  • Container: Plastic
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