It is produced to isolate cells with a density of less than 1,077 g/cm3 from human whole blood.
This product has Registration Certificate.
The preparation is clear liquid, opalescence and sediment free
pH - 7.0-7.5
osmolarity - 280±20 mOsmol/l
endotoxin content ≤0.125 EE/ml
APPLICATION:produced for isolation of lymphocytes, monocytes and neutrophils.
METHOD OF APPLICATION: Ready for use preparation. For sterile works open the vial under aseptic conditions.
For lymphocytes isolation whole blood is diluted in a ratio of 1: 1 with buffer solution of PBS Ca2 +, Md2+ free(or solution of DPBS Ca2+, Md2+ free).
After that, the diluted blood is added to Ficoll solution (at the rate of 1.5 volumes of blood per 1 volume of Ficolla solution) and centrifuged for 30 minutes at 400 g and temperature of 18-20 ° C.
After centrifugation, blood cells mononuclear fraction forms whitish meniscus at the "blood" border/Ficolla solution".
The cells are collected and washed in nutrient media for further cultivation and subsequent use for laboratory or biotechnological purposes.
HOW SUPPLIED: The preparation is in plastic or glass hermetically sealed bottles of 100 ml and 500 ml with a wide throat (Euroflacon).
SHELF LIFE: 24 months.
Shelf life after opening the primary packaging: 30 days.
STORAGE CONDITIONS: Store at temperature of +4 to +30 ° C in a place protected from light.
Transportation by all types of covered transport at similar temperature.
After opening the primary packaging, store at temperature of +2 to + 8 ° C in a place protected from light.