Cat. №: Р053
Package: 500 ml
1 700,00 ₽
0,00 ₽
+
    Delivery methods
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  • Other transport services
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Ficoll solution

It is produced  to isolate cells with a density of less than 1,077 g/cm3 from human whole blood.

This product has Registration Certificate.

The preparation is clear liquid, opalescence and sediment free

Antibiotics-free

pH - 7.0-7.5

osmolarity - 280±20 mOsmol/l

endotoxin content ≤0.125 EE/ml

APPLICATION:produced for isolation of lymphocytes, monocytes and neutrophils.

METHOD OF APPLICATION: Ready for use preparation. For sterile works open the vial under aseptic conditions.

For lymphocytes isolation whole blood is diluted in a ratio of 1: 1 with buffer solution of PBS Ca2 +, Md2+ free(or solution of DPBS Ca2+, Md2+ free).

After that, the diluted blood is added to Ficoll solution (at the rate of 1.5 volumes of blood per 1 volume of Ficolla solution) and centrifuged for 30 minutes at 400 g and temperature of 18-20 ° C.

After centrifugation, blood cells mononuclear fraction forms whitish meniscus at the "blood" border/Ficolla solution".

The cells are collected and washed in nutrient media for further cultivation and subsequent use for laboratory or biotechnological purposes.

HOW SUPPLIED: The preparation is in plastic or glass hermetically sealed bottles of 100 ml and 500 ml with a wide throat (Euroflacon).

SHELF LIFE: 24 months.

Shelf life after opening the primary packaging: 30 days.

STORAGE CONDITIONS: Store at temperature of +4 to +30 ° C in a place protected from light.

Transportation by all types of covered transport at similar temperature.

After opening the primary packaging, store at temperature of +2 to + 8 ° C in a place protected from light.

 
 
  • Package: 100 ml, 100 ml, euroflacon, 500 ml, euroflacon
  • Solution: Ficoll
  • Documents: Registration Certificate
  • Manufacturer: PanEco
  • Container: Plastic
  • Sterility Yes
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