Cat. №: СС470-02
765,00 ₽
0,00 ₽
+
The DMEM/F-12 has  excellent performance for pancreatic, certain epithelial, endothelial and granulose cell types. It can be used as a basic media for serum free cell culture.
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DMEM/F-12 powder medium, without glutamine, without bicarbonate

DMEM Ham’s F12 powder media, is a dry mixture of inorganic salts, amino acids, vitamins, glucose and phenolic red, for liquid medium preparation. Requires additional sodium bicarbonate and glutamine.

The DMEM/F-12 media are a mixture of DMEM and F-12 nutrient media in 1:1 ratio. The DMEM/F-12 medium has been used for the culture of a wide range of attached cells. It has excellent performance for pancreatic, certain epithelial, endothelial and granulose cell types. It can be used as a basic media for serum free cell culture.

This product is intended for research purposes.

Preparation instructions:

  1. Measure out 90% of final required volume of cell culture grade water. Water temperature should be 15-20°C. Mix gently, slowly add the powdered medium. Do not heat, stir until completely dissolved.
  2. Rinse original package with a small amount of water to remove all traces of powder.

  3. Add a sodium bicarbonate and glutamine* to solution. Stir until dissolved.

  4. Measure pH after complete dissolution. The pH value should be lower than the finished solution pH near 0.2 units. To adjust pH, use cultural grade solutions of 1N HCl or 1N NaOH.

  5. When the required pH is reached add additional water to bring the solution to final volume.

  6. Sterilize immediately by filtration using a membrane with a porosity of 0.22 µm (or less). Aseptically dispense medium into sterile container.

  7. The finished product should be stored at temperature of +2 to +8 °C in a dry place protected from light.

  8. Manipulate the product in aseptic conditions.
  • Attention: after filtration pH increases by 0.1-0.3 units.

* Glutamine degrades in solution during long-term storage, so it can be added sterile glutamine directly before using the media. The stable analog of glutamine is alanyl-glutamine (Сat.no. Ф102626 or Ф005) available too.

Recommended Use:

  • Do not use the product after its expiry date.
  • Dry powder medium should be free flowing. Do not use if powder caked.
  • Use gloves, mask and clothes adapted to the manipulation of the product to avoid contamination.
  • Manipulate the product in aseptic conditions. 
  • Supplements such as antibiotics or L-Glutamine should be added as sterile supplements to the ready medium.
  • Medium should be clear and free of opalescence and sediment. 

HOW SUPPLIED: The powdered mix is available in ziplock bags or plastic cans.

SHELF LIFE: 3 years.

STORAGE CONDITIONS: +2 to +8 °C. Protect the product from any form of humidity and light.

FORMULATION:

Salts and supplements

mg/l

CaCl2 x 2H2O

154,45

CuSO4 x 5H2O

0,0013

Fe(NO3)3 x 9H2O

0,05

FeSO4 x 7H2O

0,417

KCl

311,8

MgCl2

28,64

MgSO4 x 7H2O

100

NaCl

6 995,5

NaH2PO4 x H2O

62,50

Na2HPO4

71,02

ZnSO4 x 7H2O

0,43

Hypoxanthine

2,045

Lipoic acid

0,105

Linoleic acid

0,042

Putrescine 2HCl

0,081

Sodium pyruvate

55

D-glucose

3151

Phenolic Red

8,1

Amino acids

 

L-Alanine

4,45

L-Arginine

121,95

L-Asparagine H2O

7,5

L-Aspartic Acid

6,65

L-Cysteine

12,11

L-Cystine

24

L-Tyrosine

38,4

L-Glutamic Acid

7,35

Glycine

18,75

L-Histidine

23,3

L-Isoleucine

54,47

L-Leucine

59,05

L-Lysine HCl

91,25

L-Methionine

17,24

L-Phenylalanine

35,48

L-Proline

17,25

L-Serine

26,25

L-Threonine

53,45

L-Tryptophan

9,02

L-Valine

52,85

Vitamins

 

D-Ca-Pantothenate

2,24

Choline Chloride

8,98

i-Inositol

12,6

Nicotinamide

2,02

Pyridoxine HCl

2,03

Riboflavin

0,219

Thiamine HCl

2,17

Thymidine

0,365

В12

0,68

D-biotin

0,0035

Folic acid

2,65

  • Sterility -
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