Cat. №: С140Е
Manufacturer: PanEco
Volume: 450 ml
Pack size: Bottle (1 pc.)
Availability: In stock

Wholesale price:

8,48 $
0,00 $
VAT rate: 22%
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Minimal Essential Medium (MEM) is used for culture of a wide range of animal and human cells. 
    Delivery methods
  • Delivery within Russia by a transport company
  • Courier delivery in Moscow
  • Warehouse collection
  • Temperature-controlled delivery

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MEM with Hanks' Salts, with glutamine

Minimal Essential Medium (MEM) with Hanks' salts, is a ready to use mixture of inorganic salts, amino acids, vitamins, glucose and phenol red dissolved in purified water, sterilized through filters with pore size of 0.1 microns.

The MEM medium is used for the culture of a wide range of adherent cells. The media are developed on Hanks’ Balanced Salts for short term use under ambient atmospheric conditions – not for CO2 incubation.

Without Antibiotics.

This product is intended for research purposes. 

  • Appearance: clear, red-orange solution, without opalescence and sediment. 
  • pH: 7.0 to 7.4
  • Osmolarity: 290±20 mOsm/kg
  • Buffer capacity: at least 0.8 ml

PURPOSE: is used for culture of a wide range of animal and human cells.

PREPARATION INSTRUCTIONS: Ready for use. Manipulate the product in aseptic conditions.

HOW SUPPLIED: in sterile plastic bottle of 450 mL.

SHELF LIFE: 1 year.

STORAGE: +2 to +8 °C in a place protected from light.

RECOMMENDED USE:

  • Do not use the product after its expiry date.
  • Use gloves, mask and clothes adapted to the manipulation of the product to avoid contamination.
  • Manipulate the product in aseptic conditions. 
  • Supplements such as antibiotics or L-Glutamine should be added as sterile supplements to the ready medium.
  • Medium should be clear and free of opalescence and sediment. 
  • Documents: For research purposes
  • Medium: MEM
  • Glutamine: With glutamine
  • Volume: 450 ml
  • Salts: Hank's
  • HEPES: -
  • Sodium Pyruvate -
  • Sodium Bicarbonate +
Salts and supplements
  • CaCl₂ 140
  • CaCl₂·2H₂O 185.4
  • KCl 400
  • KH₂PO₄ 60
  • MgSO₄ 98
  • MgSO₄·7H₂O 200.9
  • NaCl 8000
  • Na₂HPO₄ 48
  • Na₂HPO₄·7H₂O 90.6
  • Sodium bicarbonate (NaHCO₃) 1000
  • D-Glucose 1000
  • D-Glucose·H₂O 1100
Amino acids
  • L-Arginine 103.91
  • L-Arginine HCl 126
  • L-Valine 46
  • L-Histidine 31
  • L-Isoleucine 52
  • L-Leucine 52
  • L-Lysine HCl 73
  • L-Methionine 15
  • L-Tyrosine 36.06
  • L-Threonine 48
  • L-Tryptophan 10
  • L-Phenylalanine 32
  • L-Cystine 23.77
  • L-Cystine × 2HCl 31
  • Glutamine 292
Vitamins
  • D-Calcium pantothenate 1
  • i-Inositol 2
  • Nicotinamide 1
  • Pyridoxal HCl 1
  • Riboflavin 0.1
  • Thiamine HCl 1
  • Folic acid 1
  • Choline chloride 1
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