MEM Alpha medium, without glutamine
MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, thioctic acid, vitamin B12, biotin, and ascorbic acid. MEM α – Modification can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells.
MEM α is a mixture of inorganic salts, amino acids, vitamins, glucose and phenolic red dissolved in purified water, sterilized through filters with pore size of 0.1 microns.
Without Antibiotics.
This product is intended for research purposes.
- Appearance: clear, red-orange solution, without opalescence and sediment.
- pH: 7.0 to 7.4
- Osmolarity: 300±20 mOsm/kg
- Buffer capacity: at least 3.5 ml
PURPOSE: is used for cultivation of a wide range of animal and human cells. Suitable for cultivation of attached cells.
PREPARATION INSTRUCTIONS: Before use, add 146 mg of sterile L-glutamine to a bottle with medium (450 ml). Manipulate the product in aseptic conditions.
HOW SUPPLIED: in sterile plastic bottle of 450 ml.
SHELF LIFE: 1 year.
STORAGE: +2 to +8 °C in a place protected from light.
RECOMMENDED USE:
- Do not use the product after its expiry date.
- Use gloves, mask and clothes adapted to the manipulation of the product to avoid contamination.
- Manipulate the product in aseptic conditions.
- Supplements such as antibiotics or L-Glutamine should be added as sterile supplements to the ready medium.
- Medium should be clear and free of opalescence and sediment.
