MEM with Earle's Salts, without glutamine, with HEPES
Minimal Essential Medium (MEM) is a mixture of inorganic salts, amino acids, vitamins, glucose and phenol red dissolved in purified water, sterilized through filters with pore size of 0.1 microns. The medium contains 25 mmol of HEPES and has a high buffer capacity.
Developed for cell culture in an incubator (in atmosphere of 5-7% CO2), as it is prepared on Earle's salts with a high content of sodium bicarbonate (2.2 g/l).
Without Antibiotics.
This product is intended for research purposes.
- Appearance: clear, red-orange solution, without opalescence and sediment.
- pH: 7.0 to 7.4
- Osmolarity: 280±20 mOsm/kg
- Buffer capacity: at least 7.0 mL
PURPOSE: is used for culture of a wide range of animal and human cells. Suitable for cultivation of adherent cells.
PREPARATION INSTRUCTIONS: Before use, add 146 mg of dry sterile L-glutamine to a bottle with medium (450 mL). Manipulate the product in aseptic conditions.
HOW SUPPLIED: in sterile plastic bottle of 450 mL.
SHELF LIFE: 1 year.
STORAGE: +2 to +8 °C in a place protected from light.
RECOMMENDED USE:
- Do not use the product after its expiry date.
- Use gloves, mask and clothes adapted to the manipulation of the product to avoid contamination.
- Manipulate the product in aseptic conditions.
- Supplements such as antibiotics or L-Glutamine should be added as sterile supplements to the ready medium.
- Medium should be clear and free of opalescence and sediment.
