PURPOSE
IVF 1 "PVP" medium with polyvinylpyrrolidone is developed to fix the sperm before being injected into the oocyte during the ICSI procedure.
IVF 1 "PVP" medium is used in assisted reproductive technologies, is intended for use in medical and preventive and research institutions.
THE MAIN CHARACTERISTICS:
IVF 1 "PVP" medium basis is purified water, Earl salts, glucose, polyvinylpyrrolidone. IVF 1 "PVP" medium is stabilized by HEPES. Composition includes: human serum albumin tested for the presence of hepatitis B surface antigen, for antibodies to hepatitis C viruses, HIV-1 HIV-2, for antibodies to the causative agent of syphilis.
IVF 1 "PVP" medium can be prepared both with the addition of antibiotics and phenol red, and without these components (at customer’s request). IVF 1 "PVP" medium is sterilized by filtering through filters with pore size of 0.22 microns.
APPLICATION PROTOCOL
- Before use, the closed bottle with the medium is heated to temperature of 37 ° C.
- A drop (10-15 ml) of IVF 1 "PVP" medium is placed in the center of the Petri dish.
- Depending on the number of oocytes to be used in the ICSI procedure, the required number of drops of IVF 1 "ICSI" medium aged in an incubator is placed in the same Petri dish.
- All drops with the medium are coated with liquid paraffin oil, pre-balanced in atmosphere of 5-6% CO2 at temperature of 37 ° C for 12 hours.
- Before use, a Petri dish with drops of medium should be kept for 30 minutes in a CO2 incubator at 37 ° C.
- 2 ml of prepared and washed spermatozoids are injected into a drop with IVF medium 1 "PVP". Polyvinylpyrrolidone contained in this medium helps to slow down the movement of sperm and facilitates the fixation of a single sperm in a pipette for injection.
- One sperm is fixed using an injectable micropipette. Sperm injection is recommended to be carried out in a minimum volume of IVF medium 1 "PVP".
- The procedure of sperm injection into the oocyte is performed inside drops with IVF 1 "ICSI" medium.
- Then the oocyte is fixed using a holding pipette. It is important to fix the oocyte in such a way that the polar body is in position for 6 or 12 hours.
- An injectable micropipette is injected into the oocyte at a position of 3 h. To confirm the puncture of the shiny shell, it is recommended to aspirate the cytoplasm part.
- If the pipette has penetrated into the cytoplasm, then the sperm with part of the aspirated cytoplasm is injected into the oocyte.
- After the sperm addition, pressure is applied in the holding pipette and the oocyte is disconnected.
SHELF LIFE, STORAGE AND TRANSPORTATION CONDITIONS
Shelf life is 3 months from the date of manufacture, subject to the storage regime.
Store and transport in accordance with SR 3.3.2.1248-03 at temperature of 2 to 8 ° C in a place protected from light.
Do not freeze!
Unused residue of the heated medium is not subject to reheating and use.
The product must be used within 7 days from the bottle first opening.
It is recommended to store the medium with a minimum volume of air in the bottle.
Open the vial in sterile conditions.
