IVF 1 "Ooklin" medium is developed for washing oocyte-cumulus complexes of granulosa cells and blood cells after aspiration. Aspiration of oocytes from follicles in IVF 1 "Ooklin" medium is allowed.
IVF 1 "Ooklin" medium is used in assisted reproductive technologies, intended for use in medical and preventive and research institutions.
THE MAIN CHARACTERISTICS:
The IVF 1 "Ooklin" medium basis is purified water, Earl salts, glucose, lactate, pyruvate, amino acids. IVF 1 "Ooklin" medium is stabilized by HEPES, can contain heparin, and can be prepared without it (at customer’s request). Composition includes: human serum albumin tested for the presence of hepatitis B surface antigen, for antibodies to hepatitis C viruses, HIV-1 and HIV-2, for antibodies to the causative agent of syphilis.
IVF 1 "Ooklin" medium can be prepared either with antibiotics and phenol red dye, or without these components (at customer’s request). IVF 1 "Ooklin" medium is sterilized by filtration through filters with pore size of 0.22 microns.
APPLICATION PROTOCOL
When washing oocyte-cumulus complexes in IVF1 "Ooklin" medium, the following protocol is recommended:
- Before use, a closed bottle with IVF 1 "Ooklin" medium is heated to temperature of 37 ° C. The same temperature should be maintained during oocyte washing.
- Aspirated oocyte-cumulus complexes are moved from IVF 1 "Aspiration" medium to IVF 1 "Ooklin" medium, where the washing of granulosa cells and blood cells is carried out.
- The washing of oocyte-cumulus complexes in IVF 1 "Ooklin" medium is convenient to conduct by repeatedly sequentially moving cells from one drop of the medium to another.
- After washing, the oocyte-cumulus complexes are transferred to IVF 1 "Universal" medium and kept there for 3-4 hours before fertilization or ICSI.
When carrying out oocyte aspiration in IVF 1 "Ooklin" medium, the following protocol is recommended:
- Before starting oocyte aspiration, the aspiration system is washed with 10 ml of heated IVF 1 "Ooklin" medium.
- The aspiration system is filled with IVF 1 "Ooklin" medium and due to it, follicular fluid is extracted from the follicle. Then, if necessary, the follicle is washed with a new portion of this medium, the volume of which should not exceed the amount of selected follicular fluid. In the process of oocytes aspiration, follicle size should not increase beyond its natural size.
- After the end of aspiration, the tube with aspirated oocyte-cumulus complex transferred into it is kept for a short period of time at temperature of 37 ° C.
- Then the test tube contents are transferred to a Petri dish with diameter of 60 mm, search and selection of oocyte-cumulus complexes takes place.
- The selected oocyte-cumulus complexes are transferred to the preheated to temperature of 37 ° C nutrient medium IVF 1 "Ooklin" for subsequent washing of granulosa cells and blood cells.
SHELF LIFE, STORAGE AND TRANSPORTATION CONDITIONS
Shelf life is 3 months from the date of manufacture, subject to the storage regime.
Store and transport in accordance with SR 3.3.2.1248-03 at temperature of 2 to 8 ° C in a place protected from light.
Do not freeze!
Unused residue of the heated medium is not subject to reheating and use.
The product must be used within 7 days from the bottle first opening.
It is recommended to store the medium with a minimum volume of air in the bottle.
Open the vial in sterile conditions.
