IVF 1 "Aspiration" medium is developed for aspiration of oocytes from the follicle, can be used for washing oocytes.
IVF 1 "Aspiration" medium is used in assisted reproductive technologies, intended for use in medical and preventive and research institutions.
This product has Registration Certificate.
THE MAIN CHARACTERISTICS:
The basis of IVF1 "Aspiration" medium is purified water, Earl salts, glucose. IVF1 "Aspiration" medium is stabilized by HEPES, without albumin, may contain heparin, and can be prepared without it (at customer’s request). IVF1 "Aspiration" medium can be prepared with antibiotics and phenol red dye, or without these components (at customer’s request).
The IVF1 "Aspiration" medium is sterilized by filtration through filters with pore size of 0.22 microns.
APPLICATION PROTOCOL
- Before use, the closed bottle with the medium is heated to temperature of 37 ° C. The same temperature should be maintained throughout the entire process of aspiration of oocytes from the follicle and transportation of oocytes to the laboratory. Oocytes are very sensitive to a decrease in temperature in vitro.
- Before starting oocyte aspiration, the aspiration system is washed with 10 ml of heated medium.
- The aspiration system is filled with IVF 1 "Aspiration" medium and, due to it, follicular fluid is extracted from the follicle. Then, if necessary, the follicle is washed with a new portion of this medium, the volume of which should not exceed the amount of selected follicular fluid. During the aspiration of oocytes, control that the follicle size does not increase beyond its natural size.
- After the end of aspiration, the tube with aspirated oocyte-cumulus complex transferred into it , is kept for a short period of time at temperature of 37 ° C.
- Then the contents of the test tube are transferred to a Petri dish with diameter of 60 mm and search and selection of oocyte-cumulus complexes is performed.
- The selected oocyte-cumulus complexes are transferred to the preheated to temperature of 37 ° C nutrient medium IVF 1 "Ooklin" for subsequent washing from granulosa cells and blood cells.
- Washing of oocyte-cumulus complexes is also allowed to be carried out in the IVF 1 "Aspiration" medium by sequentially moving through drops with this medium.
SHELF LIFE, STORAGE AND TRANSPORTATION CONDITIONS
Shelf life is 3 months from the date of manufacture, subject to the storage regime.
Store and transport in accordance with SR 3.3.2.1248-03 at temperature of 2 to 8 ° C in a place protected from light.
Do not freeze!
Unused residue of the heated medium is not subject to reheating and use.
The product must be used within 7 days from the bottle first opening.
It is recommended to store the medium with a minimum volume of air in the bottle.
Open the vial in sterile conditions.
