Trypsin from porcine pancreas, 100 mg, Sigma
A lyophilized powder of trypsin isolated from porcine pancreas. Trypsin is a serine protease that hydrolyses peptide bonds at the C-terminal side of lysine and arginine residues. A bioreagent suitable for cell culture and single-cell analysis. Soluble in Hanks' balanced salt solution up to 25 mg/ml.
Product: A lyophilized powder. Solubility in Hanks' balanced salt solution: 25 mg/ml. Inhibitors include DFP, TLCK, APMSF, AEBSEF and aprotinin. Solutions in 1 mM HCl are stable for 1 year at −20°C. Ca²⁺ reduces autolysis and increases stability.
Intended use: Used for tissue dissociation and detachment of adherent cells from their substrate (trypsinization), enzymatic protein hydrolysis in proteomics, and peptide digestion at trypsin:peptide ratios of 1:20–1:100. Used in cell biology, biotechnology and biochemical research.
Directions for use: For sterile work, open the bottle under aseptic conditions. Dissolve in a balanced salt solution such as Hanks' solution or PBS without Ca²⁺ or Mg²⁺ to a concentration of 0.05–0.25%. EDTA may be added to increase activity by neutralizing Ca²⁺/Mg²⁺ ions. Treat the cell monolayer at 37°C for 2–10 minutes until cells detach. Neutralize by adding serum-containing medium or a trypsin inhibitor.
Storage conditions: Store at −20°C, protected from light. Solutions in 1 mM HCl are stable for 1 year at −20°C. Transport at room temperature by any covered means of transport.
This product is intended for research use
