Liquid paraffin oil is used to cover droplets with the medium during embryo cultivation and during the ICSI procedure in order to avoid changes in the pH and temperature of the medium.
This product has Registration Certificate.
Liquid paraffin oil is used in assisted reproductive technologies, intended for use in medical and preventive and research institutions.
THE MAIN CHARACTERISTICS:
Liquid paraffin oil is sterilized by filtration through filters with pore size of 0.22 microns.
APPLICATION PROTOCOL
- The day before use, the oil should be mixed with an equal amount of IVF 1 "Universal" medium, in a sterile test tube or vial and tightly closed.
- Shake the mixture vigorously several times and leave to settle with the lid loosely closed in a CO2 incubator at 5-6% CO2 and 37 ° C.
- After separation of the phases, the oil is carefully selected into a sterile vial.
- The separation rate can be accelerated by centrifugation.
It is allowed to use liquid paraffin oil without washing with a medium.
- In this case, it is placed in an open bottle in atmosphere of 5-6% CO2 at temperature of 37 ° C overnight.
- After saturation with the gas medium, the oil is ready for use:
- The required number of drops with the medium is placed in the wells of a 4-well tablet or at the bottom of a Petri dish.
- Carefully layer the balanced paraffin oil on the drops with the medium so that the drops are completely covered with oil. SHELF LIFE, STORAGE AND TRANSPORTATION CONDITIONS
Shelf life is 3 months from the date of manufacture, subject to the storage regime.
Store and transport in accordance with SR 3.3.2.1248-03 at temperature of 2 to 8 ° C in a place protected from light.
Do not freeze!
Unused remainder of heated oil is not subject to reheating and use.
The product must be used within 7 days from the first bottle opening.
Open the vial in sterile conditions.
