Medium 199 with Earle's Salts, with glutamine
Medium 199 with Earle's Balanced Salts, with glutamine and sodium bicarbonate, is a mixture of inorganic salts, amino acids, vitamins, glucose and phenol red dissolved in purified water, sterilized through filters with pore size of 0.1 microns.
The Medium 199 was developed specifically for nutritional research of chicken fibroblasts. Today it has been used for the culture of a wide range of adherent cells. The media was developed for cell culture in an incubator (atmosphere of 5-7% CO2), as they are prepared on Earle's Balanced Salts.
Without Antibiotics.
This product is intended for research purposes.
- Appearance: clear, red-orange solution, without opalescence and sediment.
- pH: 7.0 to 7.4
- Osmolarity: 300±20 mOsm/kg
- Buffer capacity: at least 3.0 mL
PURPOSE: is used for culture of a wide range of animal and human cells. Suitable for cultivation of adherent cells.
PREPARATION INSTRUCTIONS: Ready to use. Manipulate the product in aseptic conditions.
HOW SUPPLIED: in sterile plastic bottle of 450 mL.
SHELF LIFE: 1 year.
STORAGE: +2 to +8 °C in a place protected from light.
RECOMMENDED USE:
- Do not use the product after its expiry date.
- Use gloves, mask and clothes adapted to the manipulation of the product to avoid contamination.
- Manipulate the product in aseptic conditions.
- Supplements such as antibiotics or L-Glutamine should be added as sterile supplements to the ready medium.
- Medium should be clear and free of opalescence and sediment.
