MEM with Hanks' Salts, with a double set of vitamins and amino acids
Minimal Essential Medium (MEM) with Hanks' salts, glutamine-free, with a double set of vitamins and amino acids is a mixture of inorganic salts, amino acids, vitamins, glucose and phenol red dissolved in purified water, sterilized through filters with pore size of 0.1 microns.
The MEM medium is used for the culture of a wide range of adherent cells. The media are developed on Hanks’ Balanced Salts for short term use under ambient atmospheric conditions – not for CO2 incubation.
Without Antibiotics.
This product is intended for research purposes.
- Appearance: clear, red-orange solution, without opalescence and sediment.
- pH: 7.0 to 7.4
- Osmolarity: 290±20 mOsm/kg
- Buffer capacity: at least 0.8 ml
PURPOSE: is used for culture of a wide range of animal and human cells.
PREPARATION INSTRUCTIONS: Before use, add 146 mg of dry sterile L-glutamine to a bottle with medium (450 mL). Manipulate the product in aseptic conditions.
HOW SUPPLIED: in sterile plastic bottle of 450 mL.
SHELF LIFE: 1 year.
STORAGE: +2 to +8 °C in a place protected from light.
RECOMMENDED USE:
- Do not use the product after its expiry date.
- Use gloves, mask and clothes adapted to the manipulation of the product to avoid contamination.
- Manipulate the product in aseptic conditions.
- Supplements such as antibiotics or L-Glutamine should be added as sterile supplements to the ready medium.
- Medium should be clear and free of opalescence and sediment.
