DMEM medium, without glutamine, glucose content 1 g/l, with sodium pyruvate
The Dulbecco’s Modified Eagle's Medium is used for the culture of a wide range of cells. The used of 1.0 g/l of glucose in the medium show an optimal growth for adherent cells.
DMEM media is a mixture of inorganic salts, amino acids, vitamins, glucose and phenol red dissolved in purified water, sterilized through filters with a pore size of 0.1 microns. The DMEM medium is a modification of the BME which contains a higher concentration of amino acids, vitamins and other additives.
Without Antibiotics.
This product is intended for research purposes.
- Appearance: clear, red-orange solution, without opalescence and sediment.
- pH: 7.0 to 7.4
- Osmolarity: 320±20 mOsm/kg
- Buffer capacity: at least 6.0 ml
PURPOSE: is used for culture of a wide range of animal and human cells. Suitable for cultivation of adherent cells.
PREPARATION INSTRUCTIONS: Before use, add 292 mg of dry sterile L-glutamine to a bottle with medium (450 mL). Manipulate the product in aseptic conditions.
HOW SUPPLIED: in sterile plastic bottle of 450 mL.
SHELF LIFE: 1 year.
STORAGE: +2 to +8 °C in a place protected from light.
RECOMMENDED USE:
- Do not use the product after its expiry date.
- Use gloves, mask and clothes adapted to the manipulation of the product to avoid contamination.
- Manipulate the product in aseptic conditions.
- Supplements such as antibiotics or L-Glutamine should be added as sterile supplements to the ready medium.
- Medium should be clear and free of opalescence and sediment.
